Proteasix is a web-based, peptide-centric tool dedicated to proteolytic events involved in naturally occurring peptide generation. Proteasix takes a peptide list from the user and then automatically reconstructs the N- and C- terminal cleavage sites and identifies the observed and predicted proteases involved in the proteolysis of these cleavage sites in three main species: Homo sapiens, Mus musculus and Rattus norvegicus.

Browse Proteases

"Proteases" page lists alphabetically the proteases contained in the knowledgebase. For each protein, you can access the protease description, specificity and substrates cleaved.

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Browse Substrates

"Substrates" page lists alphabetically the substrates contained in the knowledgebase. For each protein, you can access the substrate description and the proteases responsible for their cleavage.

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Search Knowledge Base

Proteases and substrates can be searched by UniProt accession or identifier, gene symbol or protein name. Cleavage site regions can be searched using the amino acid sequence.

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Prediction Tool

From an input peptide list, the tool performs an automatic reconstruction of N- and C- terminal cleavage sites and identification of endo/exoproteases involved in the proteolysis of these cleavage sites.

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Underpinning Proteasix there is the Proteasix Ontology (PxO) ontology. The PxO ontology is built in OWL and JSON-LD following the W3C’s vision of the Web of linked data. The PxO ontology is used to organise data from a variety of public sources such as UniProtKB - the UniProt Knowledgebase; MEROPS - the Peptidase Database; and CutDB - a proteolytic event database. The Proteasix KB supports the prediction tool that facilitates linking of identified protein fragments to predicted protease activity, and therefore into presumed mechanisms of disease.